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Cryo-Electron Microscopy in Dental Research

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10.52916/jmrs244134

Dr. Seyedeh Zahra Tarassoli
Department of Dentistry, University of Sechenov, Moscow, Russia.

Correspondence to: Dr. Seyedeh Zahra Tarassoli, Department of Dentistry, University of Sechenov, Moscow, Russia.
Received date: March 02, 2024; Accepted date: March 26, 2024; Published date: April 03, 2024
Citation: Dr. Tarassoli SZ. Cryo-Electron Microscopy in Dental Research J Med Res Surg. 2024;5(2):40-44. doi: 10.52916/jmrs244134
Copyright: ©2024 Dr. Tarassoli SZ. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

Abstract

The field of dental research has experienced a significant transformation with the introduction of Cryo-Electron Microscopy (Cryo-EM), a technique that aims for accuracy and originality. This innovative imaging technology has surpassed the limitations of conventional microscopy, allowing for unique understanding of the complex structures and dynamic processes within the dental microcosm. This abstract explores the significant influence of Cryo-EM in dental research, specifically highlighting its crucial role in understanding the intricacies of dental tissues, interactions between biomaterials, and the behavior of microorganisms at the nanoscale. Cryo-EM combines advanced technology and scientific investigation to not only produce detailed images but also record the dynamic movements of molecules in dental biomaterials. This allows for the development of customized dental therapies. This abstract discusses the use of Cryo-EM in studying the structure of enamel, examining the interactions between dental materials and tissues, and analyzing the complex microbial communities in the mouth. Through the clarification of these intricate particulars, Cryo-EM emerges as a revolutionary instrument, molding the field of dental research, diagnostics, and therapeutic treatments. This investigation encompasses the wonders of Cryo-EM, enhancing our comprehension of dental complexities and laying the groundwork for groundbreaking progress in oral healthcare.

Keywords:

Cryo-Electron Microscopy (Cryo-EM), Dental research, Nanoscale imaging, Dental biomaterials, Enamel structure, Oral microbiome, High-Resolution Imaging (HRI), Dental therapies, Biomaterial-Tissue Interactions (BTI), Nanoscale dynamics.

References

  1. Paine ML, White SN, Luo W, et al. Regulated gene expression dictates enamel structure and tooth function. Matrix Biol. 2001;20(5-6):273-292.
  2. Robinson C, Kirkham J, Brookes SJ, et al. The chemistry of enamel development. Int J Dev Biol. 1998;42(2):855-856.
  3. Habelitz S, Marshall SJ, Marshall GW, et al. Mechanical properties of human dental enamel on the nanometre scale. Arch Oral Biol. 2001;46(2):173-183.
  4. Tao J, Wang L, Ming GL, et al. Comparative analysis of Cryo-EM and Cryo-ET for 3D structural determination of native mammalian cells. J Struct Biol. 2019;208(1):14-20.
  5. Lo Giudice R, Nicita F, Puleio F, et al. Evaluation of periapical lesions with CBCT imaging. Int J Dent. 2019.
  6. da Silva LH, de Lima E, de Paula Miranda RB, et al. Dental ceramics: a review of new materials and processing methods. Braz Oral Res. 2017;113(8):443-465.
  7. Brookes SJ, Robinson C, Kirkham J, et al. The developing dental enamel matrix—an acid-rich, ion-poor, amelogenin-rich tissue. Eur J Oral Sci. 2001;109(s1):407-415.
  8. Weiner S, Addadi L. Design strategies in mineralized biological materials. J Mater Chem. 1999;9(8):1919-1924.
  9. Cölfen H, Antonietti M. Mesocrystals and nonclassical crystallization. In Angewandte Chemie International Edition. Wiley-VCH Verlag GmbH and Co. KGaA. 2005;44(35):pp. 5576-5591.
  10. Wopenka B, Pasteris JD. A mineralogical perspective on the apatite in bone. Mater Sci Eng C. 2005;25(2):131-143.
  11. Mangano F, Veronesi G, Hauschild U, et al. Trueness and precision of four intraoral scanners in oral implantology: a comparative in vitro study. PloS One. 2017;12(1):e0177413.
  12. Bornstein MM, Horner K, Jacobs R. Use of cone beam computed tomography in implant dentistry: current concepts, indications and limitations for clinical practice and research. Periodontol 2000. 2017;73(1):51-72.
  13. Kamburoğlu K, Kolsuz E, Murat S, et al. Accuracy of CBCT and periapical radiography in diagnosing endodontic complications. Braz Oral Res. 2019;33.
  14. Patel S, Dawood A, Wilson R, et al. The detection and management of root resorption lesions using intraoral radiography and cone beam computed tomography—an in vivo investigation. Int Endod J. 2009;42(9):831-838.
  15. Schulze RKW, Berndt D, d’Hoedt B. On cone-beam computed tomography artifacts induced by titanium implants. Clin Oral Implants Res. 2018;29(3):225-233.
  16. Loubele M, Guerrero ME, Jacobs R, et al. A comparison of jaw dimensional and quality assessments of bone characteristics with cone-beam CT, spiral tomography, and multi-slice spiral CT. Int J Oral Maxillofac Implants. 2007;22(3):446-454.
  17. Han M, Ma X, Zhang X, et al. Accuracy of digital models generated by intraoral and extraoral scanners in the assessment of oral lesions: A comparative in vitro study. J Dent. 2020;101(5):103443.
  18. Tyndall DA, Price JB, Tetradis S, et al. Position statement of the American Academy of Oral and Maxillofacial Radiology on selection criteria for the use of radiology in dental implantology with emphasis on cone beam computed tomography. Oral Surg Oral Med Oral Pathol Oral Radiol. 2012;113(6):817-826.
  19. Pauwels R, Araki K, Siewerdsen JH, et al. Technical aspects of dental CBCT: state of the art. Dentomaxillofac Radiol. 2014;44(1):20140224.
  20. Maret D, Peters OA, Vaysse F, et al. Quantitative evaluation of apical leakage of a new carrier based root canal filling material. J Biomed Mater. Res B. 2017;105(1):89-94.
  21. Zhang H, Pritzker KPH, Thibodeau EA, et al. Comparative proteomic analysis of osteoblasts on a calcium-phosphate surface. Proteomics. 2016;16(3):531-545.
  22. Pauwels R, Jacobs R, Singer SR, et al. CBCT-based bone quality assessment: are Hounsfield units applicable?. Dentomaxillofac Radiol. 2015;44(1):20140238.
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10.52916/jmrs244134
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